Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
CARTERRA INC 0.1 M MES PH 5.5
NC2232564 0.1 M MES PH 5.5
Hampton Research HR2-902-15/StockOptions HEPES #15 pH 8.2, 185 ml
HR2-902-15/StockOptions HEPES #15 pH 8.2, 185 ml. Please note items from this supplier are non-returnable.
LAB BIOREAGENTS LowBinding IV Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
Bioworld Tris-Glycine-SDS (TGS) Running Buffer (10X), 1 L
Product DescriptionTris-Glycine SDS (TGS) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris, 0.192M Glycine, and 0.1% SDS. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Teknova Tris Glycerol Buffer pH 8.0. 1000mL, Endotoxin Tested, Sterile 2 pack.
20mM Tris,25mM Sodium Chloride,2.5% Glycerol
eMolecules 199003-22-0 | tert-butyl 4-(hydroxymethyl)pyrazole-1-carboxylate | Pharmablock | MFCD24335255 | 198.222 | C9H14N2O3 | 97.000 | CC(C)(C)OC(=O)n1cc(CO)cn1 | 1g | 551300363
tert-butyl 4-(hydroxymethyl)pyrazole-1-carboxylate | Pharmablock | 199003-22-0 | MFCD24335255 | 198.222 | C9H14N2O3 | 97.000 | CC(C)(C)OC(=O)n1cc(CO)cn1 | 1g | 551300363
eMolecules 117550-36-4 | Methyl (2-(Hydroxymethyl)Phenyl)Carbamate | MFCD14680132 | 250mg
AstaTech | TERT-BUTYL 4-(CYANOMETHYL)PHENYLCARBAMATE | 1g | 449717503 | 21119 | 95.000 | 1233249-35-8 | MFCD17465121 | 232.283 | C13H16N2O2
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Alkali Scientific TRIS, Amino Buffer, Enzyme Grade, 5 Kilograms
TRIS Amino (Enzyme Grade) buffer is a high-purity solution designed specifically for enzyme-related applications in molecular biology and biochemistry. This 5kg package provides an ample supply of this reagent, which is widely used for preparing enzyme buffers in assays such as enzyme-linked immunosorbent assays (ELISA), protein assays, and PCR. The TRIS Amino buffer maintains stable pH conditions critical for optimal enzyme activity, and its enzyme-grade quality ensures minimal impurities that could interfere with biochemical reactions. This buffer is ideal for use in experiments where precise pH control is required for enzymatic reactions, as well as for preparing buffers for enzyme activity studies and protein purification.
Chemscene ChemScene | Ethyl 3-hydroxy-2-(hydroxymethyl)propanoate | 250MG | CS-B1343 | 0.95 | 99116-11-7| MFCD18909231 | 148.16
ChemScene | Ethyl 3-hydroxy-2-(hydroxymethyl)propanoate | 250MG | CS-B1343 | 0.95 | 99116-11-7| MFCD18909231 | 148.16